Anticorps Polyclonal de lapin anti-PRND

PRND Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 26947-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 26947-1-AP (same clone as 26947-1-PBS)

  Non-treated mouse testis and glucosidase treated mouse testis tissue were subjected to SDS PAGE followed by western blot with 26947-1-AP (PRND antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26947-1-PBS in a different storage buffer formulation.

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  WB analysis using 26947-1-AP (same clone as 26947-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26947-1-AP (PRND antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26947-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 26947-1-AP (same clone as 26947-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26947-1-AP (PRND antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26947-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 26947-1-AP (same clone as 26947-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26947-1-AP (PRND antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26947-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 26947-1-AP (same clone as 26947-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PRND antibody (26947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26947-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 26947-1-AP (same clone as 26947-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PRND antibody (26947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26947-1-PBS in a different storage buffer formulation.

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Informations sur le produit

26947-1-PBS cible PRND dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRND Protéine recombinante Ag25523
• Nom complet: prion protein 2 (dublet)
• Masse moléculaire calculée: 176 aa, 20 kDa
• Poids moléculaire observé: 17-20, 30 kDa
• Numéro d’acquisition GenBank: BC043644
• Symbole du gène: PRND
• Identification du gène (NCBI): 23627
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The prion gene family is mainly composed of three genes; PRNP, encoding the prion protein (PrP), PRND, encoding Doppel (Dpl), both located on the same genomic locus on mouse chromosome 2 and SPRN, encoding Shadoo (Sho), located on mouse chromosome 7. PRND (prion protein 2 also called Doppel) is a membranean N-glycosylated, glycosylphosphatidylinositol-anchored protein that is found predominantly in testis. PRND gene is found on chromosome 20, approximately 20 kbp downstream of the gene encoding cellular prionprotein, to which it is biochemically and structurally similar. 26947-1-AP antibody detects a 30 kDa glycosylated protein and a 17-20 kDa protein treated by glucosidase.

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