Anticorps Monoclonal anti-EPCR/CD201

EPCR/CD201 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1F5C4

N° de cat : 67658-1-PBS

Synonymes

CD201, EPCR, PROCR, 1F5C4, Activated protein C receptor

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Galerie de données de validation

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  WB analysis using 67658-1-Ig (same clone as 67658-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67658-1-Ig (PROCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67658-1-PBS in a different storage buffer formulation.

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  WB analysis using 67658-1-Ig (same clone as 67658-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67658-1-Ig (PROCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67658-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67658-1-Ig (same clone as 67658-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67658-1-Ig (PROCR antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67658-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67658-1-Ig (same clone as 67658-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67658-1-Ig (PROCR antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67658-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 67658-1-Ig (same clone as 67658-1-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.4 ug PROCR Monoclonal antibody (67658-1-Ig, Clone:1F5C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67658-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67658-1-PBS cible EPCR/CD201 dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EPCR/CD201 Protéine recombinante Ag25628
• Nom complet: protein C receptor, endothelial (EPCR)
• Masse moléculaire calculée: 238 aa, 27 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC014451
• Symbole du gène: PROCR
• Identification du gène (NCBI): 10544
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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