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Anticorps Polyclonal de lapin anti-CD133-1,2,4,7

CD133-1,2,4,7 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC,ELISA

Conjugaison

Non conjugué

N° de cat : 19945-1-AP

Synonymes

AC133, Antigen AC133, CD133, CD133-1,2,4,7, CORD12, MCDR2, MSTP061, PROM1, PROM1 1, PROM1 1,2,3, PROM1 1,6,7, PROM1 2, PROM1 2,3,4,5, prominin 1, Prominin like protein 1, PROML1, RP41, STGD4

Galerie de données de validation

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  • Western Blot (WB) analysis of Y79 cells using CD133-1,2,4,7 Polyclonal antibody (19945-1-AP)

    WB analysis of Y79 using 19945-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 19945-1-AP (PROM1-1,2,3,5,7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using CD133-1,2,4,7 Polyclonal antibody (19945-1-AP)

    WB analysis of mouse lung using 19945-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 19945-1-AP (PROM1-1,2,3,5,7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Y79 cells using CD133-1,2,4,7 Polyclonal antibody (19945-1-AP)

    IP experiment of Y79 using 19945-1-AP

    IP Result of anti-CD133-1,2,4,7 (IP:19945-1-AP, 4ug; Detection:19945-1-AP 1:300) with Y79 cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CD133-1,2,4,7 Polyclonal antibody (19945-1-AP)

    IHC staining of human gliomas using 19945-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19945-1-AP (CD133-1,2,4,7 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CD133-1,2,4,7 Polyclonal antibody (19945-1-AP)

    IHC staining of human gliomas using 19945-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19945-1-AP (CD133-1,2,4,7 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Applications testées

Résultats positifs en WBcellules Y79, tissu pulmonaire de souris
Résultats positifs en IPcellules Y79
Résultats positifs en IHCtissu de gliome humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

19945-1-AP cible CD133-1,2,4,7 dans les applications de WB, IP, IHC,ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet prominin 1
Masse moléculaire calculée 97 kDa
Poids moléculaire observé 115 kDa
Numéro d’acquisition GenBankNM_001145851
Symbole du gène PROM1
Identification du gène (NCBI) 8842
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors. This antibody recognizes transcript variant 1,2,4,7 (UNIPROT has updated the info of isoforms)of CD133.

Protocole

Product Specific Protocols
WB protocol for CD133-1,2,4,7 antibody 19945-1-APDownload protocol
IHC protocol for CD133-1,2,4,7 antibody 19945-1-APDownload protocol
IP protocol for CD133-1,2,4,7 antibody 19945-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols