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Anticorps Polyclonal de lapin anti-CD133-1,2

CD133-1,2 Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP,ELISA

Conjugaison

Non conjugué

N° de cat : 19946-1-AP

Synonymes

AC133, Antigen AC133, CD133, CORD12, MCDR2, MSTP061, PROM1, PROM1 1, PROM1 1,2, PROM1 1,2,3,5,7, PROM1 1,6,7, PROM1 2, PROM1 2,3,4,5, prominin 1, Prominin like protein 1, PROML1, RP41, STGD4

Galerie de données de validation

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  • Western Blot (WB) analysis of HT-29 cells using CD133-1,2 Polyclonal antibody (19946-1-AP)

    WB analysis of HT-29 using 19946-1-AP

    HT-29 cells were subjected to SDS PAGE followed by western blot with 19946-1-AP (CD133-1,2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Caco-2 cells using CD133-1,2 Polyclonal antibody (19946-1-AP)

    WB analysis of Caco-2 using 19946-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 19946-1-AP (CD133-1,2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Y79 cells using CD133-1,2 Polyclonal antibody (19946-1-AP)

    IP experiment of Y79 using 19946-1-AP

    IP Result of anti-CD133-1,2,3 (IP:19946-1-AP, 4ug; Detection:19946-1-AP 1:300) with Y79 cells lysate 2000ug.

Applications testées

Résultats positifs en WBcellules HT-29, cellules Caco-2
Résultats positifs en IPcellules Y79

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

19946-1-AP cible CD133-1,2 dans les applications de WB, IP,ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet prominin 1
Masse moléculaire calculée 97 kDa
Poids moléculaire observé 110-120 kDa
Numéro d’acquisition GenBankNM_006017
Symbole du gène PROM1
Identification du gène (NCBI) 8842
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors. This antibody recognizes transcript variant 1,2 of CD133.

Protocole

Product Specific Protocols
WB protocol for CD133-1,2 antibody 19946-1-APDownload protocol
IP protocol for CD133-1,2 antibody 19946-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols