Anticorps Polyclonal de lapin anti-CD133

CD133 Polyclonal Antibody for FC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 18495-1-AP

Synonymes

AC133, Antigen AC133, CD133, CORD12, MCDR2, MSTP061, PROM1, PROM1 1, PROM1 1,2,3, PROM1 1,2,3,5,7, PROM1 1,6,7, PROM1 2, PROM1 2,3,4,5, prominin 1, Prominin like protein 1, PROML1, RP41, STGD4

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Galerie de données de validation

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  WB analysis using 18495-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18495-1-AP (CD133 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 18495-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18495-1-AP (PROM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 18495-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 18495-1-AP (CD133 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 18495-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 18495-1-AP (CD133 Antibody) at dilution of 1:200 (under 40x lens).

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  FC experiment of HT-29 using 18495-1-AP

  1X10^6 HT-29 cells were stained with 0.20 ug Anti-Human CD133 (18495-1-AP) (red) or 0.20 ug isotype control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HT-29, tissu de muscle squelettique de souris
• Résultats positifs en cytométrie: cellules HT-29

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

18495-1-AP cible CD133 dans les applications de WB, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD133 Protéine recombinante Ag13327
• Nom complet: prominin 1
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 110-115 kDa
• Numéro d’acquisition GenBank: BC012089
• Symbole du gène: PROM1
• Identification du gène (NCBI): 8842
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors.

Publications

Species	Application	Title
human,mouse	WB,IF	J Oncol; The Role of Purine Metabolism-Related Genes PPAT and IMPDH1 in the Carcinogenesis of Intrahepatic Cholangiocarcinoma Based on Metabonomic and Bioinformatic Analyses; Authors - Chang-Jun Liu
human	WB	Cancer Med; Integrated analysis based on vesicle trafficking-related genes identifying CNIH4 as a novel therapeutic target for glioma; Authors - Zhen Fang
human	IF	Oncol Lett; Regulation of the migration of colorectal cancer stem cells via the TLR4/MyD88 signaling pathway by the novel surface marker CD14 following LPS stimulation; Authors - Yufei Li

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