Anticorps Polyclonal de lapin anti-CD133

CD133 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 18495-1-AP

Synonymes

PROM1, MSTP061, MCDR2, CORD12, Antigen AC133

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Galerie de données de validation

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  WB analysis using 18495-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18495-1-AP (CD133 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 18495-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18495-1-AP (PROM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 18495-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 18495-1-AP (CD133 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 18495-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 18495-1-AP (CD133 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 18495-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 18495-1-AP (CD133 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 18495-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 18495-1-AP (CD133 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HT-29, tissu de muscle squelettique de souris
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 2 publications below

Informations sur le produit

18495-1-AP cible CD133 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD133 Protéine recombinante Ag13327
• Nom complet: prominin 1
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 110-115 kDa
• Numéro d’acquisition GenBank: BC012089
• Symbole du gène: CD133
• Identification du gène (NCBI): 8842
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors.

Publications

Species	Application	Title
human	WB	Front Oncol; USP7 regulates growth and maintains the stemness of p53-mutant colorectal cancer cells via stabilizing of mutant p53; Authors - Xue Li
human,mouse	WB,IF	J Oncol; The Role of Purine Metabolism-Related Genes PPAT and IMPDH1 in the Carcinogenesis of Intrahepatic Cholangiocarcinoma Based on Metabonomic and Bioinformatic Analyses; Authors - Chang-Jun Liu
human	WB	Cancer Med; Integrated analysis based on vesicle trafficking-related genes identifying CNIH4 as a novel therapeutic target for glioma; Authors - Zhen Fang
human	IF	Oncol Lett; Regulation of the migration of colorectal cancer stem cells via the TLR4/MyD88 signaling pathway by the novel surface marker CD14 following LPS stimulation; Authors - Yufei Li
human	WB	World J Oncol; DNA Damage-Induced Apoptosis Suppressor Triggers Progression and Stemness of Glioma by Enhancing Lymphoid Enhancer-Binding Factor 1 Expression; Authors - You Lin Chen
human,mouse	WB	Bioorg Med Chem; Synthesis and anti-tumor activity of menthylamine derivatives: Focusing on the potent inhibitory effect of compound W8 on glioma growth; Authors - Xiaoran Wu

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