Anticorps Monoclonal anti-PROX1

PROX1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D4B1

N° de cat : 67438-1-PBS

Synonymes

1D4B1, Homeobox prospero-like protein PROX1, prospero homeobox 1, Prospero homeobox protein 1, PROX-1

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Galerie de données de validation

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  WB analysis of HuH-7 using 67438-1-Ig (same clone as 67438-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 67438-1-Ig (PROX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 67438-1-Ig (same clone as 67438-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67438-1-Ig (PROX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67438-1-Ig (same clone as 67438-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67438-1-Ig (PROX1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67438-1-Ig (same clone as 67438-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67438-1-Ig (PROX1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

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  IF Staining of HuH-7 using 67438-1-Ig (same clone as 67438-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using PROX1 antibody (67438-1-Ig, Clone: 1D4B1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67438-1-PBS cible PROX1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PROX1 Protéine recombinante Ag9681
• Nom complet: prospero homeobox 1
• Masse moléculaire calculée: 737 aa, 83 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC024201
• Symbole du gène: PROX1
• Identification du gène (NCBI): 5629
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PROX1, Prospero homeobox protein 1, belongs to the Prospero homeobox family and contain a Prospero-type homeobox DNA-binding domain. PROX1 plays a fundamental role in early development of CNS and regulates the expression of gene and development of postmitotic, undifferentiated neurons. Also, PROX1 is a required regulator of the development of the lymphatic system. This antibody is raised against part of C-terminal chain of human PROX1. The calculated molecular weight of PROX1 is 83 kDa, but with post-translational modifications, PROX1 is about 90 kDa.

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