Anticorps Polyclonal de lapin anti-PRPF3

PRPF3 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 10106-1-AP

Synonymes

RP18, Prp3p, PRP3, Pre-mRNA-splicing factor 3, HPRP3P

---

Galerie de données de validation

• 

  WB analysis of Y79 using 10106-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10106-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10106-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10106-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10106-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10106-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 10106-1-AP

  IP result of anti-PRPF3 (IP:10106-1-AP, 5ug; Detection:10106-1-AP 1:500) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human stomach using 10106-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10106-1-AP (PRPF3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules Y79, cellules HepG2, cellules L02, tissu cérébral de souris, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

10106-1-AP cible PRPF3 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRPF3 Protéine recombinante Ag0154
• Nom complet: PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae)
• Masse moléculaire calculée: 78 kDa
• Poids moléculaire observé: 90 kDa, 78 kDa
• Numéro d’acquisition GenBank: BC000184
• Symbole du gène: PRPF3
• Identification du gène (NCBI): 9129
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The pre-mRNA splicing occurs in spliceosomes, which consist of four ribonucleoprotein (snRNP) particles (U1, U2, U5 and U4/U6) and more than fifty proteins. This U4/U6-associated splicing factor, Hprp3p, is a 77 kDa protein, which is homologous to the Saccharomyces cerevisiae splicing factor Prp3p and may play a role in spliceosome assembly. Western blot analysis of HeLa cell nuclear extracts detected PRP3 protein at an apparent molecular mass of about 90 kD(PMID: 9328476 ). The discrepancy between the molecular mass reported here and that predicted from the Hprp3p coding sequence can be attributed, at least partially, to the highly positive charge; its value of predicted isoelectric point (IP) is ∼9.99. This antibody is a rabbit polyclonal antibody raised against an internal region of human PRPF3. A predicted molecular mass of 77 kDa and the gene is named PRPF3 because it is homologous to the yeast PRP3 gene. We noticed that although the predicted molecular mass of PRPF3 is77 kDa, the protein produced in E.coli migrated like a 90 kDa protein in SDS-PAGE. (PMID: 9328476)

Publications

Species	Application	Title
human	WB	Hum Mol Genet; PRPF mutations are associated with generalized defects in spliceosome formation and pre-mRNA splicing in patients with retinitis pigmentosa.; Authors - Tanackovic Goranka G
human	WB	Mol Cell Biol; A quantitative high-throughput in vitro splicing assay identifies inhibitors of spliceosome catalysis.; Authors - Berg Michael G MG
human	WB	J Biol Chem; PRPF19 regulates p53-dependent cellular senescence by modulating alternative splicing of MDM4 mRNA.; Authors - Kimiyoshi Yano
human	IF	Cell Mol Biol Lett; TMEM43 promotes pancreatic cancer progression by stabilizing PRPF3 and regulating RAP2B/ERK axis.; Authors - Junqiang Li
mouse	WB	Food Funct; Flaxseed oil attenuates PFOS-induced testicular damage by regulating RNA alternative splicing; Authors - Jingyi Ren

{{ptg:RelatedPrimaryAntibodies}}