Anticorps Polyclonal de lapin anti-PRPF31

PRPF31 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27750-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 27750-1-AP (same clone as 27750-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27750-1-AP (PRPF31 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27750-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 27750-1-AP (same clone as 27750-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 27750-1-AP (PRPF31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27750-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 27750-1-AP (same clone as 27750-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 27750-1-AP (PRPF31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27750-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 27750-1-AP (same clone as 27750-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using PRPF31 antibody (27750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27750-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 27750-1-AP (same clone as 27750-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using PRPF31 antibody (27750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27750-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27750-1-PBS cible PRPF31 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRPF31 Protéine recombinante Ag26716
• Nom complet: PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae)
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: NM_015629
• Symbole du gène: PRPF31
• Identification du gène (NCBI): 26121
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRPF31 is a component of the spliceosome complex. It is recruited to introns following the attachment of U4 and U6 RNAs and the 15.5K protein. The addition of PRPF31 is crucial for the transition of the spliceosomal complex to the activated state.

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