Anticorps Recombinant de lapin anti-PRPF31

PRPF31 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251005H8

N° de cat : 86315-2-PBS

Synonymes

hPrp31, NY BR 99, Protein 61K, PRP31, RP11

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Galerie de données de validation

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  WB analysis using 86315-2-RR (same clone as 86315-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86315-2-RR (PRPF31 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86315-2-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 86315-2-RR (same clone as 86315-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PRPF31 antibody (86315-2-RR, Clone: 251005H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86315-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86315-2-RR (same clone as 86315-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRPF31 antibody (86315-2-RR, Clone: 251005H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86315-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86315-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86315-2-RR against Human PRPF31 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

86315-2-PBS cible PRPF31 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PRPF31 Protéine recombinante Ag26716
• Nom complet: PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae)
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: NM_015629
• Symbole du gène: PRPF31
• Identification du gène (NCBI): 26121
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRPF31 is a component of the spliceosome complex. It is recruited to introns following the attachment of U4 and U6 RNAs and the 15.5K protein. The addition of PRPF31 is crucial for the transition of the spliceosomal complex to the activated state.

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