Anticorps Polyclonal de lapin anti-PRPF6

• Phare
• Validé par KD/KO

PRPF6 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 23929-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HEK-293 using 23929-1-AP (same clone as 23929-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 23929-1-AP (same clone as 23929-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 23929-1-AP (same clone as 23929-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 23929-1-AP (same clone as 23929-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 23929-1-AP (same clone as 23929-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 23929-1-AP (same clone as 23929-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 23929-1-AP (same clone as 23929-1-PBS)

  IP result of anti-PRPF6 (IP:23929-1-AP, 4ug; Detection:23929-1-AP 1:300) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 23929-1-AP (same clone as 23929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23929-1-AP (PRPF6 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 23929-1-AP (same clone as 23929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23929-1-AP (PRPF6 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

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Informations sur le produit

23929-1-PBS cible PRPF6 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRPF6 Protéine recombinante Ag13817
• Nom complet: PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae)
• Masse moléculaire calculée: 941 aa, 107 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC001666
• Symbole du gène: PRPF6
• Identification du gène (NCBI): 24148
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRPF6 is involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, it can enhance dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation.

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