Anticorps Polyclonal de lapin anti-PRPF8

PRPF8 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11171-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of Jurkat using 11171-1-AP (same clone as 11171-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 11171-1-AP (same clone as 11171-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 11171-1-AP (same clone as 11171-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11171-1-AP (same clone as 11171-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11171-1-AP (same clone as 11171-1-PBS)

  IP result of anti-PRPF8 (IP:11171-1-AP, 4ug; Detection:11171-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 11171-1-AP (same clone as 11171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 11171-1-AP (same clone as 11171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 11171-1-AP (same clone as 11171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11171-1-PBS cible PRPF8 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRPF8 Protéine recombinante Ag1652
• Nom complet: PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae)
• Masse moléculaire calculée: 274 kDa
• Poids moléculaire observé: 220 kDa
• Numéro d’acquisition GenBank: BC064370
• Symbole du gène: PRPF8
• Identification du gène (NCBI): 10594
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRPF8, a 220kd protein,is a component of mammalian spliceosomes, large multiprotein complexes involved in the removal of introns from mRNA precursor. It has a central structural role in the architecture of the U5 small nuclear ribonucleoprotein (snRNP) particle.

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