Anticorps Polyclonal de lapin anti-PRPSAP2

PRPSAP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 17814-1-AP

Synonymes

PAP41, PRPSAP2

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Galerie de données de validation

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  WB analysis using 17814-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17814-1-AP (PRPSAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17814-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17814-1-AP (PRPSAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 17814-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 17814-1-AP (PRPSAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 17814-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17814-1-AP (PRPSAP2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human pancreas using 17814-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17814-1-AP (PRPSAP2 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of BxPC-3 using 17814-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using PRPSAP2 antibody (17814-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules BxPC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

17814-1-AP cible PRPSAP2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRPSAP2 Protéine recombinante Ag12132
• Nom complet: phosphoribosyl pyrophosphate synthetase-associated protein 2
• Masse moléculaire calculée: 369 aa, 41 kDa
• Poids moléculaire observé: 37-41 kDa
• Numéro d’acquisition GenBank: BC106050
• Symbole du gène: PRPSAP2
• Identification du gène (NCBI): 5636
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRPSAP2, also named as PAP41, is 369 amino acid protein, which Seems to play a negative regulatory role in 5-phosphoribose 1-diphosphate synthesis. PRPSAP2 is another protein involved in nucleic acid metabolism. The PRPSAP2 gene encodes part of the enzyme PRPP synthetase, which catalyzes the formation of phosphoribosylpyrophosphate, being a primary substrate for newly formed purine and pyrimidine nucleotides. Depletion of the PRPP synthetase causes growth arrest], the reverse has not been shown. Overexpression of SHMT1 and PRPSAP2 (and also COPS3) has been reported to occur in multiple myelomas . PRPSAP2 exists several isoforms with the range of molecular weight about 35-43 kDa.

Publications

Species	Application	Title
mouse	WB	bioRxiv; Evolutionary origins and innovations sculpting the mammalian PRPS enzyme complex; Authors - Bibek R Karki ; KO Validated
human	WB	bioRxiv; PRPS activity tunes redox homeostasis in Myc-driven lymphoma; Authors - Austin C MacMillan

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