Anticorps Polyclonal de lapin anti-PRTN3

PRTN3 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 25278-1-AP

Synonymes

ACPA, AGP7, C ANCA, C ANCA antigen, Leukocyte proteinase 3, MBN, MBT, Myeloblastin, Neutrophil proteinase 4, P29, PR 3, PR3, proteinase 3, PRTN3, Wegener autoantigen

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Galerie de données de validation

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  WB analysis of human placenta using 25278-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 25278-1-AP (PRTN3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 25278-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 25278-1-AP (PRTN3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 25278-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25278-1-AP (PRTN3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 25278-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25278-1-AP (PRTN3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 25278-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 25278-1-AP (PRTN3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 25278-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 25278-1-AP (PRTN3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25278-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25278-1-AP (PRTN3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 25278-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25278-1-AP (PRTN3 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain,
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer du côlon humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

25278-1-AP cible PRTN3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRTN3 Protéine recombinante Ag16765
• Nom complet: proteinase 3
• Masse moléculaire calculée: 256 aa, 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC096183
• Symbole du gène: PRTN3
• Identification du gène (NCBI): 5657
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IHC	Biomedicines; Comparative Proteomic Analysis of tPVAT during Ang II Infusion.; Authors - Xiuying Liang
human	WB	Front Cell Neurosci; Meningitic Escherichia coli-Induced Interleukin-17A Facilitates Blood-Brain Barrier Disruption via Inhibiting Proteinase 3/Protease-Activated Receptor 2 Axis.; Authors - Bojie Xu
human	WB	Toxicol In Vitro; Activation of inflammasomes by tyrosine kinase inhibitors of vascular endothelial growth factor receptor: Implications for VEGFR TKIs-induced immune related adverse events.; Authors - Hideki Imano

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Malgorzata (Verified Customer) (08-08-2022)

The antibody worked on my positive control - cell lysate from cultured THP-1 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: THP-1

PRTN3 Antibody Western Blot validation (1000 dilution) in THP-1 (Cat no:25278-1-AP)