Anticorps Polyclonal de lapin anti-PRDX3

• Phare
• Validé par KD/KO

PRDX3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(34)

Avis (1)

N° de cat : 10664-1-AP

Synonymes

peroxiredoxin 3, MER5, EC:1.11.1.24, AOP-1, AOP1

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Galerie de données de validation

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  WB analysis using 10664-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10664-1-AP (PRDX3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10664-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10664-1-AP (PRDX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10664-1-AP

  WB result of PRDX3 antibody (10664-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX3 transfected HEK-293 cells.

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  WB analysis using 10664-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10664-1-AP (PRDX3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 10664-1-AP

  WB result from Qing-Xi Yue, (PMID:20449493); “Control” and “GAD-treated” above the panel represent the control cells and HeLa cells treated with 10 μMGAD for 48 h, respectively. Each blot is the representative result of three independent experiments.

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  WB analysis of MCF-7 using 10664-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10664-1-AP (PRDX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10664-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10664-1-AP (PRDX3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10664-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10664-1-AP (PRDX3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10664-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRDX3 antibody (10664-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules LNCaP, cellules MCF-7, tissu cardiaque de souris, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 30 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

10664-1-AP cible PRDX3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRDX3 Protéine recombinante Ag1062
• Nom complet: peroxiredoxin 3
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 26-28 kDa
• Numéro d’acquisition GenBank: BC007062
• Symbole du gène: PRDX3
• Identification du gène (NCBI): 10935
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells (PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.

Publications

Species	Application	Title
human,mouse	WB	Nat Commun; Inhibited peroxidase activity of peroxiredoxin 1 by palmitic acid exacerbates nonalcoholic steatohepatitis in male mice; Authors - Wen Yin
human	WB	Redox Biol; Icariin alleviates uveitis by targeting peroxiredoxin 3 to modulate retinal microglia M1/M2 phenotypic polarization.; Authors - Guoqing Wang ; KD Validated
mouse	WB	Redox Biol; PMID: 32604037; Authors - Anindya Roy Chowdhury
human	WB,IHC	Redox Biol; LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis.; Authors - Wen-Su Wei
rat	IF	Oxid Med Cell Longev; Paeoniflorin Upregulates Mitochondrial Thioredoxin of Schwann Cells to Improve Diabetic Peripheral Neuropathy Indicated by 4D Label-Free Quantitative Proteomics.; Authors - Xinwei Yang
human	WB	Mol Cell Proteomics; Proteomics characterization of the cytotoxicity mechanism of ganoderic acid D and computer-automated estimation of the possible drug target network.; Authors - Yue Qing-Xi QX

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Guillermo (Verified Customer) (09-03-2022)

I trust in Proteintech, my favourite antibody company. 5/5 product.

• Applications: Western Blot

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