Anticorps Monoclonal anti-PRX5

• Phare
• Validé par KD/KO

PRX5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B8C5

N° de cat : 67599-1-PBS

Synonymes

Peroxiredoxin 5, PRDX5, 1B8C5, ACR 1, ACR1

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Galerie de données de validation

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  WB analysis using 67599-1-Ig (same clone as 67599-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67599-1-Ig (PRX5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67599-1-PBS in a different storage buffer formulation.

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  WB analysis using 67599-1-Ig (same clone as 67599-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67599-1-Ig (PRX5 antibody) at dilution of 1:5200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67599-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67599-1-Ig (same clone as 67599-1-PBS)

  WB result of PRX5 antibody (67599-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRX5 transfected HepG2 cells. This data was developed using the same antibody clone with 67599-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67599-1-Ig (same clone as 67599-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67599-1-Ig (PRX5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67599-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67599-1-Ig (same clone as 67599-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67599-1-Ig (PRX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67599-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67599-1-Ig (same clone as 67599-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRX5 antibody (67599-1-Ig, Clone: 1B8C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67599-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67599-1-PBS cible PRX5 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PRX5 Protéine recombinante Ag12262
• Nom complet: peroxiredoxin 5
• Masse moléculaire calculée: 214 aa, 22 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC110983
• Symbole du gène: PRX5
• Identification du gène (NCBI): 25824
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Peroxiredoxin 5 (Prx5) is a member of a family consisting of six antioxidant enzymes. Prx5 is ubiquitously expressed in various tissues including mitochondria and peroxisomes, implying that Prx5 functions as a regulator of the cellular oxidation state. Prx5 plays a critical role in protecting cells from oxidative stress by inhibiting the accumulation of reactive oxygen species and cell death. Prx5 has an intensive ROS scavenging activity because it is located in the cytosol and mitochondria. Prx5 expression was upregulated during adipogenesis and Prx5 overexpression suppressed adipogenesis by regulating cytosolic and mitochondrial ROS generation. (PMID: 29777756, PMID: 31505325, PMID: 22020876)

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