Anticorps Monoclonal anti-PSAP

• Phare
• Validé par KD/KO

PSAP Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F11B2

N° de cat : 66473-1-PBS

Synonymes

CSAct, Component C, Co beta glucosidase, Cerebroside sulfate activator, A1 activator

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Galerie de données de validation

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  WB analysis of human milk using 66473-1-Ig (same clone as 66473-1-PBS)

  human milk were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66473-1-Ig (same clone as 66473-1-PBS)

  WB result of PSAP antibody (66473-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HeLa cells. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66473-1-Ig (same clone as 66473-1-PBS)

  WB result of PSAP antibody (66473-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HepG2 cells. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66473-1-Ig (same clone as 66473-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 66473-1-Ig (same clone as 66473-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66473-1-Ig (PSAP antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66473-1-Ig (PSAP antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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  IF Staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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  IF Staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66473-1-Ig (same clone as 66473-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66473-1-PBS cible PSAP dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PSAP Protéine recombinante Ag1194
• Nom complet: prosaposin
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC001503
• Symbole du gène: PSAP
• Identification du gène (NCBI): 5660
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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