Anticorps Recombinant de lapin anti-PSAP

PSAP Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250312D3

N° de cat : 86043-2-RR

Synonymes

Prosaposin, Saposin-A, Saposin-B, Saposin-B-Val, Saposin-C

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Galerie de données de validation

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  WB analysis using 86043-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86043-2-RR (Psap antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 86043-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using PSAP antibody (86043-2-RR, Clone: 250312D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules RAW 264.7, tissu de cervelet de souris, tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86043-2-RR cible PSAP dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PSAP Protéine recombinante Eg3155
• Nom complet: prosaposin
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: NM_011179.3
• Symbole du gène: Psap
• Identification du gène (NCBI): 19156
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Prosaposin (PSAP) is also named as Sulfated glycoprotein 1 (SGP-1). PSAP is a secreted neurotrophic factor and a lysosomal protein serving as the precursor of saposins (A-D), which are cofactors for hydrolases of sphingolipids (PMID: 25130661, PMID: 31220974). PSAP modulates glycosphingolipid metabolism and variants have been linked to Parkinson's disease (PD) (PMID: 37726325). As a dual-function protein, PSAP is a lysosome-associated protein that participates in a variety of cellular processes. In the lysosome, PSAP is processed to activate enzymes that degrade lipids. PSAP proteins located extracellularly are involved in cancer progression, such as proliferation and tumor death suppression signaling (PMID: 39405903). Prosaposin (PSAP) has two forms: a precursor and a secreted form. The secreted form has neurotrophic, myelinotrophic, and myotrophic properties. The precursor form is a precursor protein of saposins A-D (PMID: 33242172). Both human and mouse prosaposin are 65-70 kDa proteins cleaved in the lysosomes into four 10-15 kDa proteins designated saposins A, B, C and D (PMID: 8573994). Sulfated glycoprotein-1 (prosaposin) exists in 2 forms: a 65 kDa form targeted to lysosomes and a post-translationally modified to the 70 kDa form secreted extracellularly (PMID: 8593668).

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