Anticorps Polyclonal de lapin anti-PSD95

PSD95 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(13)

Avis (2)

N° de cat : 30255-1-AP

Synonymes

Disks large homolog 4, DLG4, FLJ97752, FLJ98574, PSD 95, PSD95, SAP 90, SAP90, Synapse associated protein 90

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Galerie de données de validation

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  WB analysis using 30255-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30255-1-AP (PSD95 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 30255-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30255-1-AP (PSD95 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IF: See 1 publications below

Informations sur le produit

30255-1-AP cible PSD95 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSD95 Protéine recombinante Ag33000
• Nom complet: discs, large homolog 4 (Drosophila)
• Masse moléculaire calculée: 81 kDa
• Poids moléculaire observé: 90-95 kDa
• Numéro d’acquisition GenBank: NM_001365
• Symbole du gène: PSD95
• Identification du gène (NCBI): 1742
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSD-95 (postsynaptic density protein 95) also known as SAP-90 (synapse-associated protein 90) is a protein that in humans is encoded by the DLG4 (disks large homolog 4) gene. PSD-95 is a scaffolding protein of the MAGUK protein family, and engages in several vital protein-protein interactions in the brain with its PDZ domains. It has been suggested that PSD-95 is composed of two supramodules, one of which is the PDZ1-2 tandem domain. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation. Observed MW of PSD95 is 90-95 kDa due to phosphorylation (PMID: 20682303).

Publications

Species	Application	Title
mouse	WB	J Ethnopharmacol; Lancao decoction alleviates Alzheimer's disease: depending on activating CaMKII to protect neuronal refunction by reducing β-amyloid in the hippocampus; Authors - Lei Wu
mouse	WB	FASEB J; HDAC1/2/3-mediated downregulation of neurogranin is involved in cognitive impairment in offspring exposed to maternal subclinical hypothyroidism; Authors - Xi Yu
mouse	WB	Actas Esp Psiquiatr; Exercise-induced Activation of SIRT1/BDNF/mTORC1 Signaling Pathway: A Novel Mechanism to Reduce Neuroinflammation and Improve Post-stroke Depression; Authors - Junze Tang
mouse	WB	Environ Pollut; Bisphenol F exposure induces depression-like changes: Roles of the kynurenine metabolic pathway along the "liver-brain" axis; Authors - Zheng Yu
mouse	WB	Neurobiol Stress; Effects of autotaxin and lysophosphatidic acid deficiencies on depression-like behaviors in mice exposed to chronic unpredictable mild stress; Authors - Chao Wang
mouse	WB	Aging (Albany NY); Chronic stress induces Alzheimer's disease-like pathologies through DNA damage-Chk1-CIP2A signaling; Authors - Zhuoqun Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Olga (Verified Customer) (10-03-2025)

You can surely dilute it more than 1:1000, as the signal was already saturated at 2.5 seconds of exposition. I find it better for mouse samples as it does not give non-specificity in non-brain tissues (comparing to 20665-1-AP PSD95 antibody) - all the mouse cells tested are of neuronal origin. The principal band size depends on protein marker used (see picture) - and can vary from 80 to 110 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse tissues and cell lines: spleen, heart, lung, liver, forebrain, primary cerebellar neurons, Neuro2A, mHypo

PSD95 Antibody Western Blot validation (1:1000 dilution) in mouse tissues and cell lines: spleen, heart, lung, liver, forebrain, primary cerebellar neurons, Neuro2A, mHypo (Cat no:30255-1-AP)

FH

Alessandro (Verified Customer) (02-04-2025)

high specificity and sensitivity, providing reliable and reproducible results

• Applications: Immunohistochemistry, Immunofluorescence

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