Anticorps Polyclonal de lapin anti-PSD95-Specific,DLG4

• Phare
• Validé par KD/KO

PSD95-Specific,DLG4 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

154

Avis (3)

N° de cat : 20665-1-AP

Synonymes

Disks large homolog 4, DLG4, PSD 95, PSD95, PSD95-Specific,DLG4, SAP 90, SAP90, Synapse associated protein 90

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Galerie de données de validation

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  WB analysis of HeLa using 20665-1-AP

  WB result of PSD95-Specific,DLG4 antibody (20665-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSD95-Specific,DLG4 transfected HeLa cells.

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  WB analysis using 20665-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20665-1-AP (PSD95-Specific,DLG4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20665-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20665-1-AP (PSD95-Specific,DLG4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20665-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20665-1-AP (PSD95-Specific,DLG4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Neuro-2a using 20665-1-AP

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human PSD95-Specific,DLG4 (20665-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules Neuro-2a,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 132 publications below
• IF: See 30 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

20665-1-AP cible PSD95-Specific,DLG4 dans les applications de WB, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: discs, large homolog 4 (Drosophila)
• Masse moléculaire calculée: 85 kDa
• Poids moléculaire observé: 90-95 kDa
• Numéro d’acquisition GenBank: NM_001365
• Symbole du gène: DLG4
• Identification du gène (NCBI): 1742
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSD-95 (postsynaptic density protein 95) also known as SAP-90 (synapse-associated protein 90) is a protein that in humans is encoded by the DLG4 (disks large homolog 4) gene. PSD-95 is a scaffolding protein of the MAGUK protein family, and engages in several vital protein-protein interactions in the brain with its PDZ domains. It has been suggested that PSD-95 is composed of two supramodules, one of which is the PDZ1-2 tandem domain. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation. Observed MW of PSD95 is 90-95 kDa due to phosphorylation (PMID: 20682303).

Publications

Species	Application	Title
mouse		Nat Neurosci; Foxp2 controls synaptic wiring of corticostriatal circuits and vocal communication by opposing Mef2c.; Authors - Yi-Chuan Chen
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
mouse	WB,IF	Neuron; Selective removal of astrocytic APOE4 strongly protects against tau-mediated neurodegeneration and decreases synaptic phagocytosis by microglia.; Authors - Chao Wang
mouse	WB	Sci Adv; Loss of schizophrenia-related miR-501-3p in mice impairs sociability and memory by enhancing mGluR5-mediated glutamatergic transmission; Authors - Wenquan Liang
mouse	WB	Sci Adv; RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission; Authors - Pengcheng Ma
mouse	WB	Mol Psychiatry; A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.; Authors - Yue Qin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (03-11-2023)

Works well in IHC and WB in mouse brain. WB: 4% milk in TBST and semi-dry transfer; detects a double band at ~100kDa. IHC: 4% PFA fixed and paraffinized sections; standard antigen retrieval and DAB detection. Used at 1:1000, with incubation overnight at 4C.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse, CNS tissue

PSD95-Specific,DLG4 Antibody Western Blot,Immunohistochemistry validation (1:1000 dilution) in Mouse, CNS tissue (Cat no:20665-1-AP)

FH

Lisa (Verified Customer) (03-17-2022)

The antibody works well for WB

• Applications: Western Blot
• Primary Antibody Dilution: 1:1500
• Cell Tissue Type: hiPSCs-derived neurons

FH

James (Verified Customer) (09-29-2021)

Antibody was not as specific as I had hoped. I used the antibody at 1:1000, I detected a band at 95kDa which is PSD-95, however, other bands were also detected. I will be looking to purchase a more specific monoclonal antibody for my next experiments as the specificity is an issue for me.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Neurogenin 2 iPSC Neurons Day 21

PSD95-Specific,DLG4 Antibody Western Blot validation (1:1000 dilution) in Neurogenin 2 iPSC Neurons Day 21 (Cat no:20665-1-AP)

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