Anticorps Recombinant de lapin anti-GINS2

GINS2 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241870E9

N° de cat : 84486-2-RR

Synonymes

PSF2, Pfs2, HSPC037, GINS 2, DC5

---

Galerie de données de validation

• 

  WB analysis using 84486-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84486-2-RR (GINS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 84486-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GINS2 antibody (84486-2-RR, Clone: 241870E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of Jurkat using 84486-2-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug GINS2 Recombinant antibody (84486-2-RR, Clone:241870E9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 84486-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84486-2-RR against Human GINS2 were performed. The affinity constant is 0.82 nM.

---

Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84486-2-RR cible GINS2 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: GINS2 Protéine recombinante Ag9180
• Nom complet: GINS complex subunit 2 (Psf2 homolog)
• Masse moléculaire calculée: 185 aa, 21 kDa
• Poids moléculaire observé: 20-25 kDa
• Numéro d’acquisition GenBank: BC010164
• Symbole du gène: GINS2
• Identification du gène (NCBI): 51659
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GINS2, also named as DNA replication complex GINS protein PSF2, CGI-122, DC5, or HSPC037, is a 185 amino acid protein, which belongs to the GINS2/PSF2 family. The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA. GINS2 is a part of the the GINS complex, an integral component of the replicative helicase machinery that facilitates progression of the replisome complex.

{{ptg:RelatedPrimaryAntibodies}}