Anticorps Monoclonal anti-PSIP1

PSIP1 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1E9E7

N° de cat : 67723-1-Ig

Synonymes

Lens epithelium-derived growth factor, LEDGF, DFS70, CLL-associated antigen KW-7, CLL associated antigen KW 7

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Galerie de données de validation

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  WB analysis using 67723-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67723-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67723-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 67723-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67723-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 67723-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSIP1 antibody (67723-1-Ig, Clone: 1E9E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules U2OS
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67723-1-Ig cible PSIP1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PSIP1 Protéine recombinante Ag30562
• Nom complet: PC4 and SFRS1 interacting protein 1
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: NM_021144
• Symbole du gène: PSIP1
• Identification du gène (NCBI): 11168
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSIP1, also known as PC4 and SRSF1 Interacting Protein 1 or Lens Epithelium-Derived Growth Factor (LEDGF), is a transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. It also plays a role in lens epithelial cell gene regulation and stress responses. It is a chromatin-associated protein with histone chaperone activity, involved in transcriptional elongation. PSIP1 may act as an adapter to coordinate pre-mRNA splicing. PSIP1 encodes two protein isoforms with molecular masses of 52 and 75 kDa. p52 and p75 were identified as interacting with transcription factor PC4 and shown to act as transcriptional coactivators.

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