Anticorps Recombinant de lapin anti-PSIP1

PSIP1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242841C5

N° de cat : 85395-1-RR

Synonymes

242841C5, CLL associated antigen KW 7, CLL-associated antigen KW-7, DFS70, LEDGF

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Galerie de données de validation

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  WB analysis using 85395-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85395-1-RR (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 85395-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 85395-1-RR (PSIP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 85395-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 85395-1-RR (PSIP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 85395-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85395-1-RR (PSIP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 85395-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85395-1-RR (PSIP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 85395-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSIP1 antibody (85395-1-RR, Clone: 242841C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85395-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85395-1-RR against Human PSIP1 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules HEK-293T, cellules K-562, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85395-1-RR cible PSIP1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PSIP1 Protéine recombinante Ag21886
• Nom complet: PC4 and SFRS1 interacting protein 1
• Masse moléculaire calculée: 530 aa, 60 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC064135
• Symbole du gène: PSIP1
• Identification du gène (NCBI): 11168
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSIP1, also known as PC4 and SRSF1 Interacting Protein 1 or Lens Epithelium-Derived Growth Factor (LEDGF), is a transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. It also plays a role in lens epithelial cell gene regulation and stress responses. It is a chromatin-associated protein with histone chaperone activity, involved in transcriptional elongation. PSIP1 may act as an adapter to coordinate pre-mRNA splicing. PSIP1 encodes two protein isoforms with molecular masses of 52 and 75 kDa. p52 and p75 were identified as interacting with transcription factor PC4 and shown to act as transcriptional coactivators.

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