Anticorps Monoclonal anti-PSMA/GCPII

PSMA/GCPII Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3G4E12

N° de cat : 66678-1-PBS

Synonymes

FOLH1, PSMA, 3G4E12, Cell growth-inhibiting gene 27 protein, EC:3.4.17.21

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Galerie de données de validation

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  WB analysis of LNCaP using 66678-1-Ig (same clone as 66678-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66678-1-Ig (FOLH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

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  WB analysis using 66678-1-Ig (same clone as 66678-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

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  WB analysis of rat kidney using 66678-1-Ig (same clone as 66678-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 66678-1-Ig (FOLH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66678-1-Ig (same clone as 66678-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66678-1-Ig (same clone as 66678-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66678-1-Ig (same clone as 66678-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 66678-1-Ig (same clone as 66678-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 66678-1-Ig (same clone as 66678-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

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  IF Staining of human prostate cancer using 66678-1-Ig (same clone as 66678-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSMA/GCPII antibody (66678-1-Ig, Clone: 3G4E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 66678-1-Ig (same clone as 66678-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using PSMA/GCPII antibody (66678-1-Ig, Clone: 3G4E12 ) at dilution of 1:90 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 66678-1-Ig (same clone as 66678-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using PSMA/GCPII antibody (66678-1-Ig, Clone: 3G4E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51112-1

  Cytometric bead array standard curve of MP51112-1, PSMA/FOLH1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66678-2-PBS. Detection antibody: 66678-1-PBS. Standard:Ag16594. Range: 1.563-100 ng/mL.

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  Affinity and Kinetic Characterization of 66678-1-Ig

  Biolayer interferometry (BLl) kinetic assay of 66678-1-Ig against Human PSMA/GCPII was performed. The affinity constant is below 1 pM.

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Informations sur le produit

66678-1-PBS cible PSMA/GCPII dans les applications de WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PSMA/GCPII Protéine recombinante Ag16594
• Nom complet: folate hydrolase (prostate-specific membrane antigen) 1
• Masse moléculaire calculée: 719 aa, 81 kDa
• Poids moléculaire observé: 100-120 kDa
• Numéro d’acquisition GenBank: BC025672
• Symbole du gène: PSMA
• Identification du gène (NCBI): 2346
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.

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