Anticorps Monoclonal anti-PSMA1

PSMA1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D1D5

N° de cat : 67910-1-PBS

Synonymes

30 kDa prosomal protein, HC2, Macropain subunit C2, NU, PROS 30, PROS30, Proteasome component C2, Proteasome nu chain, PSC2, PSMA1

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Galerie de données de validation

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  WB analysis using 67910-1-Ig (same clone as 67910-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67910-1-Ig (PSMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67910-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 67910-1-Ig (same clone as 67910-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67910-1-Ig (PSMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67910-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67910-1-Ig (same clone as 67910-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67910-1-Ig (PSMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67910-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67910-1-Ig (same clone as 67910-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67910-1-Ig (PSMA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67910-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67910-1-Ig (same clone as 67910-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67910-1-Ig (PSMA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67910-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67910-1-PBS cible PSMA1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PSMA1 Protéine recombinante Ag30527
• Nom complet: proteasome (prosome, macropain) subunit, alpha type, 1
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 29-33 kDa
• Numéro d’acquisition GenBank: BC015105
• Symbole du gène: PSMA1
• Identification du gène (NCBI): 5682
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PSMA1(Proteasome subunit alpha type-1) is also named as HC2, NU, PROS30, PSC2 and belongs to the peptidase T1A family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It is induced in breast cancer tissue and up-regulated in liver tumor tissues(PMID:17127214;16317774;17004105).

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