Anticorps Polyclonal de lapin anti-PSMA3

PSMA3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11887-1-PBS

Synonymes

PSC8, Proteasome subunit alpha type-3, HC8

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Galerie de données de validation

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  WB analysis of A549 using 11887-1-AP (same clone as 11887-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

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  WB analysis of human lung using 11887-1-AP (same clone as 11887-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-12 using 11887-1-AP (same clone as 11887-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11887-1-AP (same clone as 11887-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 11887-1-AP (same clone as 11887-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 11887-1-AP (same clone as 11887-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 11887-1-AP (same clone as 11887-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 11887-1-AP (same clone as 11887-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11887-1-AP (same clone as 11887-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11887-1-AP (same clone as 11887-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA3 antibody (11887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11887-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11887-1-PBS cible PSMA3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMA3 Protéine recombinante Ag2495
• Nom complet: proteasome (prosome, macropain) subunit, alpha type, 3
• Masse moléculaire calculée: 255 aa, 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC029402
• Symbole du gène: PSMA3
• Identification du gène (NCBI): 5684
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PSMA3(Proteasome subunit alpha type-3) is also named as HC8, PSC8 and belongs to the peptidase T1A family. The proteasome is a multicatalytic protease complex that catalyzes an energy-dependent, extralysosomal proteolytic pathway responsible for selective elimination of proteins with aberrant structures and naturally occurring short-lived proteins related to metabolic regulation and cell cycle progression. It has 2 isoforms produced by alternative splicing.

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