Anticorps Polyclonal de lapin anti-PSMA5

• Phare
• Validé par KD/KO

PSMA5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31384-1-PBS

Synonymes

Proteasome subunit alpha type-5, PSC5, ZETA

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Galerie de données de validation

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  WB analysis using 31384-1-AP (same clone as 31384-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31384-1-AP (PSMA5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31384-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 31384-1-AP (same clone as 31384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31384-1-AP (PSMA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31384-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 31384-1-AP (same clone as 31384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31384-1-AP (PSMA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31384-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 31384-1-AP (same clone as 31384-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSMA5 antibody (31384-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 31384-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31384-1-PBS cible PSMA5 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMA5 Protéine recombinante Ag34928
• Nom complet: proteasome (prosome, macropain) subunit, alpha type, 5
• Masse moléculaire calculée: 241 aa, 26 kDa
• Poids moléculaire observé: 26-30 kDa
• Numéro d’acquisition GenBank: BC103751
• Symbole du gène: PSMA5
• Identification du gène (NCBI): 5686
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Proteasome subunit alpha type-5(PSMA5) is a component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. It binds to two 19S regulatory particles(RP) to form the 26S proteasome, an ATP-dependent multisubunit protease that degrades polyubiquitinated proteins into small peptides.(PMID: 26661102)

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