Anticorps Recombinant de lapin anti-PSMA5

PSMA5 Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241629H2

N° de cat : 84424-4-PBS

Synonymes

241629H2, Proteasome subunit alpha type-5, PSC5, ZETA

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Galerie de données de validation

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  IHC staining of mouse liver using 84424-4-RR (same clone as 84424-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84424-4-RR (PSMA5 antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 84424-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84424-4-RR (same clone as 84424-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84424-4-RR (PSMA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84424-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84424-4-RR (same clone as 84424-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA5 antibody (84424-4-RR, Clone: 241629H2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84424-4-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 84424-4-RR (same clone as 84424-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSMA5 antibody (84424-4-RR, Clone: 241629H2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84424-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84424-4-RR (same clone as 84424-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA5 antibody (84424-4-RR, Clone: 241629H2 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 84424-4-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 84424-4-RR (same clone as 84424-4-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PSMA5 Recombinant antibody (84424-4-RR, Clone:241629H2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84424-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01286-3

  Cytometric bead array standard curve of MP01286-3, PSMA5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84424-4-PBS. Detection antibody: 84424-1-PBS. Standard: Ag34928. Range: 0.156-20 ng/mL.

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  Affinity and Kinetic Characterization of 84424-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84424-4-RR against Human PSMA5 were performed. The affinity constant is 36.6 pM.

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Informations sur le produit

84424-4-PBS cible PSMA5 dans les applications de IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PSMA5 Protéine recombinante Ag34928
• Nom complet: proteasome (prosome, macropain) subunit, alpha type, 5
• Masse moléculaire calculée: 241 aa, 26 kDa
• Numéro d’acquisition GenBank: BC103751
• Symbole du gène: PSMA5
• Identification du gène (NCBI): 5686
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Proteasome subunit alpha type-5(PSMA5) is a component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. It binds to two 19S regulatory particles(RP) to form the 26S proteasome, an ATP-dependent multisubunit protease that degrades polyubiquitinated proteins into small peptides.(PMID: 26661102)

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