Anticorps Polyclonal de lapin anti-PSMB5

• Phare
• Validé par KD/KO

PSMB5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(16)

N° de cat : 19178-1-AP

Synonymes

X, Proteasome subunit beta type-5, MB1, LMPX, EC:3.4.25.1

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Galerie de données de validation

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  WB analysis using 19178-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19178-1-AP (PSMB5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19178-1-AP

  WB result of PSMB5 antibody (19178-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMB5 transfected HeLa cells.

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  IHC staining of human breast cancer using 19178-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 19178-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19178-1-AP (PSMB5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 19178-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 19178-1-AP (PSMB5 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules L02
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

19178-1-AP cible PSMB5 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMB5 Protéine recombinante Ag5828
• Nom complet: proteasome (prosome, macropain) subunit, beta type, 5
• Masse moléculaire calculée: 263 aa, 28 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC057840
• Symbole du gène: PSMB5
• Identification du gène (NCBI): 5693
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMB5(Proteasome subunit beta type-5) is also named as LMPX, MB1, X and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.RNAi-mediated silencing of.It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Cell; Cancer vulnerabilities unveiled by genomic loss.; Authors - Nijhawan Deepak D
human	WB	Nat Commun; The deubiquitinase OTUD1 regulates immunoglobulin production and proteasome inhibitor sensitivity in multiple myeloma; Authors - Alexander Vdovin
human	IF,WB	Cancer Lett; Combinations of ivermectin with proteasome inhibitors induce synergistic lethality in multiple myeloma; Authors - Hongmei Luo
mouse	WB	Front Pharmacol; Selective Inhibition of the Immunoproteasome β5i Prevents PTEN Degradation and Attenuates Cardiac Hypertrophy.; Authors - Xin Xie
human	WB	J Cancer; Cytoplasm-localized SIRT1 downregulation attenuates apoptosis and cell cycle arrest in cisplatin-resistant lung cancer A549 cells.; Authors - Hyeran Yu
mouse	WB	Biochem Pharmacol; Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP); Authors - Xin Xie

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