Anticorps Recombinant de lapin anti-PSMB6

PSMB6 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251232G5

N° de cat : 86491-1-PBS

Synonymes

EC:3.4.25.1, LMPY, Proteasome subunit beta type-6, Y

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Galerie de données de validation

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  WB analysis using 86491-1-RR (same clone as 86491-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86491-1-RR (PSMB6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86491-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86491-1-RR (same clone as 86491-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMB6 antibody (86491-1-RR, Clone: 251232G5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86491-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86491-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86491-1-RR against Human PSMB6 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

86491-1-PBS cible PSMB6 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PSMB6 Protéine recombinante Ag2296
• Nom complet: proteasome (prosome, macropain) subunit, beta type, 6
• Masse moléculaire calculée: 239 aa, 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC000835
• Symbole du gène: PSMB6
• Identification du gène (NCBI): 5694
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PSMB6(Proteasome subunit beta type-6) is also named as LMPY, Y and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It may also catalyzes basal processing of intracellular antigens.It can be up-regulated in anaplastic thyroid cancer cell lines and down-regulated by IFNG/IFN-gamma (at protein level)(PMID:8066462;15613457).

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