Anticorps Polyclonal de lapin anti-PSMB7

PSMB7 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 14727-1-AP

Synonymes

20S proteasome, Macropain chain Z, Proteasome subunit beta type 7, Proteasome subunit Z, PSMB7

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Galerie de données de validation

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  WB analysis of Raji using 14727-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14727-1-AP (PSMB7 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 14727-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14727-1-AP (PSMB7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14727-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14727-1-AP (PSMB7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 14727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14727-1-AP (PSMB7 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 14727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14727-1-AP (PSMB7 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 14727-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14727-1-AP (PSMB7 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14727-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14727-1-AP (PSMB7 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 14727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 14727-1-AP (PSMB7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 14727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 14727-1-AP (PSMB7 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules A549
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du sein humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

14727-1-AP cible PSMB7 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMB7 Protéine recombinante Ag6486
• Nom complet: proteasome (prosome, macropain) subunit, beta type, 7
• Masse moléculaire calculée: 29.9 kDa
• Poids moléculaire observé: 25-27 kDa
• Numéro d’acquisition GenBank: BC000509
• Symbole du gène: PSMB7
• Identification du gène (NCBI): 5695
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. PSMB7 protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. Component of the 20S core proteasome complex are involved in the proteolytic degradation of most intracellular proteins. PSMB7 displays a trypsin-like activity.(PMID:15244466, PMID:27176742, PMID: 8610016)

Publications

Species	Application	Title
mouse	WB	J Clin Invest; Androgen receptor polyglutamine expansion drives age-dependent quality control defects and muscle dysfunction.; Authors - Samir R Nath
rat	WB	Int J Mol Sci; Impaired 26S Proteasome Assembly Precedes Neuronal Loss in Mutant UBQLN2 Rats.; Authors - Wenjuan Zhang
mouse	WB	Biochem Pharmacol; Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP); Authors - Xin Xie