Anticorps Polyclonal de lapin anti-PSMB8

PSMB8 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 14859-1-AP

Synonymes

beta5i, D6S216, D6S216E, LMP7, Low molecular mass protein 7, Macropain subunit C13, Proteasome component C13, Proteasome subunit beta 5i, Proteasome subunit beta type 8, PSMB5i, PSMB8, RING10, Y2

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Galerie de données de validation

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  WB analysis of mouse kidney using 14859-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 14859-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14859-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14859-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14859-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 14859-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14859-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14859-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14859-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14859-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14859-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14859-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14859-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14859-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14859-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14859-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 14859-1-AP

  IP Result of anti-LMP7 (IP:14859-1-AP, 3ug; Detection:14859-1-AP 1:1000) with Raji cells lysate 2800ug.

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  IHC staining of human lymphoma using 14859-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14859-1-AP (LMP7 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 14859-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14859-1-AP (LMP7 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 14859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMB8 antibody (14859-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 14859-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug LMP7 antibody (14859-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules Jurkat, cellules Raji, tissu rénal humain
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IHC: tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Informations sur le produit

14859-1-AP cible PSMB8 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMB8 Protéine recombinante Ag6660
• Nom complet: proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC001114
• Symbole du gène: PSMB8
• Identification du gène (NCBI): 5696
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMB8(Proteasome subunit beta type-8) is also named as LMP7, PSMB5i, RING10, Y2 and belongs to the peptidase T1B family. The gene encodes the chymotrypsin-like catalytic subunit of the immunoproteasome(PMID: 19525961). PSMB8 has a role in controlling pathogenic immune responses and may be a target in autoimmune disorders. Its prosequence is not essential for incorporation of PSMB8 into the maturing proteasome, but it increased the efficiency of PSMB8 incorporation and proteasome maturation(PMID: 10926487). The pro-PSMB8 is a 276aa protein with the molecular mass of 30 kDa, and the mature form is about 23kDa due to the 72aa propeptide cleaved. Defects in PSMB8 are the cause of Nakajo syndrome (NKJO).

Publications

Species	Application	Title
human	WB	J Cell Mol Med; miR-451a inhibits cancer growth, epithelial-mesenchymal transition and induces apoptosis in papillary thyroid cancer by targeting PSMB8.; Authors - Xinlong Fan
mouse	WB,IHC	J Biol Chem; PITHD1 is a proteasome-interacting protein essential for male fertilization.; Authors - Hiroyuki Kondo
mouse	WB	JCI Insight; Blockade of the CXCR3/CXCL10 axis ameliorates inflammation caused by immunoproteasome dysfunction.; Authors - Yuki Sasaki
human,mouse	WB	Sci Rep; Airway epithelial immunoproteasome subunit LMP7 protects against rhinovirus infection; Authors - Kris Genelyn Dimasuay ; KO Validated
		Int Immunopharmacol; Targeted inhibition of the immunoproteasome blocks endothelial MHC class II antigen presentation to CD4+ T cells in chronic liver injury; Authors - Yuwei Zhang
mouse	WB	J Neuroinflammation; β-arrestin1 regulates astrocytic reactivity via Drp1-dependent mitochondrial fission: implications in postoperative delirium; Authors - Fuzhou Hua