Anticorps Monoclonal anti-PSMB8

PSMB8 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

2A5B6

N° de cat : 66759-1-Ig

Synonymes

beta5i, D6S216, D6S216E, LMP7, Low molecular mass protein 7, Macropain subunit C13, Proteasome component C13, Proteasome subunit beta 5i, Proteasome subunit beta type 8, PSMB5i, PSMB8, RING10, Y2

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Galerie de données de validation

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  WB analysis using 66759-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66759-1-Ig (PSMB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66759-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66759-1-Ig (PSMB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66759-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66759-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 66759-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 66759-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 66759-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 66759-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 66759-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66759-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66759-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung cancer using 66759-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using PSMB8 antibody (66759-1-Ig, Clone: 2A5B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human liver cancer using 66759-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using PSMB8 antibody (66759-1-Ig, Clone: 2A5B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 66759-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMB8 antibody (66759-1-Ig, Clone: 2A5B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of C2C12 using 66759-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using PSMB8 antibody (66759-1-Ig, Clone: 2A5B6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HSC-T6, cellules Raji, cellules Ramos
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer de l'œsophage humain, tissu de cancer du foie humain, tissu de côlon de rat, tissu de côlon de souris, tissu d'estomac de rat, tissu d'estomac de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules C2C12, cellules HeLa, tissu de cancer du foie humain, tissu de cancer du poumon humain

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66759-1-Ig cible PSMB8 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PSMB8 Protéine recombinante Ag6780
• Nom complet: proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC001114
• Symbole du gène: PSMB8
• Identification du gène (NCBI): 5696
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMB8(Proteasome subunit beta type-8) is also named as LMP7, PSMB5i, RING10, Y2 and belongs to the peptidase T1B family. The gene encodes the chymotrypsin-like catalytic subunit of the immunoproteasome(PMID: 19525961). PSMB8 has a role in controlling pathogenic immune responses and may be a target in autoimmune disorders. Its prosequence is not essential for incorporation of PSMB8 into the maturing proteasome, but it increased the efficiency of PSMB8 incorporation and proteasome maturation(PMID: 10926487). The pro-PSMB8 is a 276aa protein with the molecular mass of 30 kDa, and the mature form is about 23kDa due to the 72aa propeptide cleaved. Defects in PSMB8 are the cause of Nakajo syndrome (NKJO).