Anticorps Recombinant de lapin anti-PSMC1

PSMC1 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251014B9

N° de cat : 86237-1-PBS

Synonymes

26S proteasome AAA-ATPase subunit RPT2, 26S proteasome regulatory subunit 4, P26S4

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Galerie de données de validation

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  WB analysis using 86237-1-RR (same clone as 86237-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86237-1-RR (PSMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86237-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 86237-1-RR (same clone as 86237-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 86237-1-RR (PSMC1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86237-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 86237-1-RR (same clone as 86237-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PSMC1 antibody (86237-1-RR, Clone: 251014B9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86237-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86237-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86237-1-RR against Human PSMC1 were performed. The affinity constant is 0.402 nM.

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Informations sur le produit

86237-1-PBS cible PSMC1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PSMC1 Protéine recombinante Ag1677
• Nom complet: proteasome (prosome, macropain) 26S subunit, ATPase, 1
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 52-60 kDa
• Numéro d’acquisition GenBank: BC016368
• Symbole du gène: PSMC1
• Identification du gène (NCBI): 5700
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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