Anticorps Polyclonal de lapin anti-PSMC3IP

• Phare
• Validé par KD/KO

PSMC3IP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 11339-1-AP

Synonymes

PSMC3-interacting protein, PSMC3 interacting protein, Proteasome 26S ATPase subunit 3-interacting protein, HOP2, Homologous-pairing protein 2 homolog

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Galerie de données de validation

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  WB analysis using 11339-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11339-1-AP (HOP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 11339-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11339-1-AP (HOP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 11339-1-AP

  IP result of anti-HOP2 (IP:11339-1-AP, 4ug; Detection:11339-1-AP 1:500) with Jurkat cells lysate 3200ug.

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  IHC staining of mouse testis using 11339-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11339-1-AP (HOP2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 11339-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11339-1-AP (HOP2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 11339-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11339-1-AP (HOP2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 11339-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11339-1-AP (HOP2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11339-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HOP2 antibody (11339-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat, cellules K-562
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu testiculaire de souris, tissu testiculaire de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

11339-1-AP cible PSMC3IP dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMC3IP Protéine recombinante Ag1883
• Nom complet: PSMC3 interacting protein
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 24-29 kDa
• Numéro d’acquisition GenBank: BC008792
• Symbole du gène: PSMC3IP
• Identification du gène (NCBI): 29893
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMC3IP, also called HOP2, encodes a protein that functions in meiotic recombination. It is a subunit of the PSMC3IP/MND1 complex, which interacts with PSMC3/TBP1 to stimulate DMC1- and RAD51-mediated strand exchange during meiosis. The protein encoded by this gene can also co-activate ligand-driven transcription mediated by estrogen, androgen, glucocorticoid, progesterone, and thyroid nuclear receptors. Mutations in this gene cause XX female gonadal dysgenesis.

Publications

Species	Application	Title
human	WB	Cell; Interchromosomal Homology Searches Drive Directional ALT Telomere Movement and Synapsis.; Authors - Nam Woo Cho ; KD Validated
mouse	IF	Nucleic Acids Res; RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis; Authors - Chao Liu
human	WB	Cell Rep; MND1 and PSMC3IP control PARP inhibitor sensitivity in mitotic cells; Authors - Anabel Zelceski
human		Nat Commun; Promotion of homology-directed DNA repair by polyamines.; Authors - Chih-Ying Lee
human	WB,IF	Oncotarget; A study of meiomitosis and novel pathways of genomic instability in cutaneous T-cell lymphomas (CTCL).; Authors - Matthew Tsang

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