Anticorps Recombinant de lapin anti-PSMD1

PSMD1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240458C9

N° de cat : 83521-4-RR

Synonymes

PSMD 1, 26S proteasome non-ATPase regulatory subunit 1, 240458C9

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Galerie de données de validation

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  WB analysis using 83521-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83521-4-RR (PSMD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83521-4-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83521-4-RR (PSMD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 83521-4-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83521-4-RR (PSMD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 83521-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMD1 antibody (83521-4-RR, Clone: 240458C9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of MCF-7 using 83521-4-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PSMD1 Recombinant antibody (83521-4-RR, Clone:240458C9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83521-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83521-4-RR against Human PSMD1 were performed. The affinity constant is 74.2 pM.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules MCF-7, cellules SKOV-3, tissu cérébral de rat, tissu cérébral de souris, tissu testiculaire de souris
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83521-4-RR cible PSMD1 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PSMD1 Protéine recombinante Ag12009
• Nom complet: proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
• Masse moléculaire calculée: 953 aa, 106 kDa
• Poids moléculaire observé: 106 kDa
• Numéro d’acquisition GenBank: BC094720
• Symbole du gène: PSMD1
• Identification du gène (NCBI): 5707
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMD1 is a component of the 26S proteasome and contributes to its function regulation. PSMD1 performs an important role in the regulation of numerous cellular processes through the degradation of proteins targeted for breakdown through ubiquitin. PSMD1 has been found to be closely related to a variety of cancers such as lung adenocarcinoma and breast cancer.

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