Anticorps Recombinant de lapin anti-PSMD10/Gankyrin

PSMD10/Gankyrin Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250170D7

N° de cat : 86120-2-PBS

Synonymes

PSMD10, 26S proteasome non-ATPase regulatory subunit 10, Gankyrin, p28, p28(GANK)

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Galerie de données de validation

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  WB analysis using 86120-2-RR (same clone as 86120-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86120-2-RR (PSMD10/Gankyrin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86120-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 86120-2-RR (same clone as 86120-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86120-2-RR (PSMD10/Gankyrin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86120-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 86120-2-RR (same clone as 86120-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86120-2-RR (PSMD10/Gankyrin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86120-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86120-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86120-2-RR against Human PSMD10/Gankyrin were performed. The affinity constant is 28.2 nM.

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Informations sur le produit

86120-2-PBS cible PSMD10/Gankyrin dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PSMD10/Gankyrin Protéine recombinante Ag2994
• Nom complet: proteasome (prosome, macropain) 26S subunit, non-ATPase, 10
• Masse moléculaire calculée: 226 aa, 24 kDa
• Poids moléculaire observé: 24-28 kDa
• Numéro d’acquisition GenBank: BC011960
• Symbole du gène: PSMD10
• Identification du gène (NCBI): 5716
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PSMD10, also known as Gankyrin, is a subunit of the 19S regulatory particle of the proteasome and plays a crucial role in protein degradation. It is a small, 25 kDa cytoplasmic-nuclear shuttling protein, highly conserved throughout evolution (~40% identity to yeast Nas6p). PSMD10 is involved in the regulation of cell cycle progression and apoptosis by interacting with key proteins such as p53 and Rb. PSMD10 has been identified as an oncoprotein, promoting tumor cell proliferation and survival in various cancers (PMID: 16581249, PMID: 31576540).

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