Anticorps Recombinant de lapin anti-PSMD11

PSMD11 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251499B5

N° de cat : 86596-3-PBS

Synonymes

26S proteasome non-ATPase regulatory subunit 11, p44.5, Rpn6, S9

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Galerie de données de validation

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  WB analysis using 86596-3-RR (same clone as 86596-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86596-3-RR (PSMD11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86596-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 86596-3-RR (same clone as 86596-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 86596-3-RR (PSMD11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86596-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86596-3-RR (same clone as 86596-3-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PSMD11 antibody (86596-3-RR, Clone: 251499B5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86596-3-PBS in a different storage buffer formulation.

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Informations sur le produit

86596-3-PBS cible PSMD11 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PSMD11 Protéine recombinante Ag6435
• Nom complet: proteasome (prosome, macropain) 26S subunit, non-ATPase, 11
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC000437
• Symbole du gène: PSMD11
• Identification du gène (NCBI): 5717
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The 26 S proteasome is a 2.5-MDa molecular machine that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core particle and two 19 S regulatory particles (RPs) composed of 6 ATPase (RPT) and 13 non-ATPase (RPN) subunits. PSMD11 gene encodes 19S proteasome subunit RPN6. Increased levels of PSMD11 and a corresponding increased assembly of the 26S/30S proteasome is correlated with high proteasome activity. In vitro ectopic expression of PSMD11 is sufficient to increase proteasome assembly and activity. Latest research has implicated PSMD11 in regulation of human embryonic stem cells function.

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