Anticorps Recombinant de lapin anti-PSMD14/POH1

• Phare
• Validé par KD/KO

PSMD14/POH1 Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

242160E7

N° de cat : 84767-3-PBS

Synonymes

PSMD14, 242160E7, 26S proteasome non-ATPase regulatory subunit 14, 26S proteasome-associated PAD1 homolog 1, EC:3.4.19.-

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Galerie de données de validation

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  WB analysis using 84767-3-RR (same clone as 84767-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84767-3-RR (PSMD14/POH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84767-3-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 84767-3-RR (same clone as 84767-3-PBS)

  WB result of PSMD14/POH1 antibody (84767-3-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD14/POH1 transfected A431 cells. This data was developed using the same antibody clone with 84767-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01566-3

  Sandwich ELISA standard curve of MP01566-3, Human PSMD14/POH1 Recombinant Matched Antibody Pair - PBS only. 84767-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2694. 84767-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP01566-3

  The mean PSMD14/POH1 concentration was determined to be 12.08 ng/mL in A431 cell extract based on a 1.30 mg/mL extract load, 24.32 ng/mL in K-562 cell extract based on a 2.40 mg/mL extract load and 10.64 ng/mL in HT-1080 cell extract based on a 1.40 mg/mL extract load.

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  IHC staining of mouse heart using 84767-3-RR (same clone as 84767-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84767-3-RR (PSMD14/POH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84767-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 84767-3-RR (same clone as 84767-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84767-3-RR (PSMD14/POH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84767-3-PBS in a different storage buffer formulation.

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  IF Staining of A375 using 84767-3-RR (same clone as 84767-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using PSMD14/POH1 antibody (84767-3-RR, Clone: 242160E7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84767-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84767-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 84767-3-PBS against Human PSMD14/POH1 was performed. The affinity constant is 3.45 nM.

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  Affinity and Kinetic Characterization of 84767-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84767-3-RR against Human PSMD14/POH1 were performed. The affinity constant is 3.60 nM.

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Informations sur le produit

84767-3-PBS cible PSMD14/POH1 dans les applications de WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PSMD14/POH1 Protéine recombinante Ag2694
• Nom complet: proteasome (prosome, macropain) 26S subunit, non-ATPase, 14
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC009524
• Symbole du gène: PSMD14
• Identification du gène (NCBI): 10213
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The PSMD14 (POH1, also known as Rpn11/MPR1/S13/CepP1) protein is a metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. PSMD14 is highly expressed in the heart and skeletal muscle. In carcinoma cell lines. down-regulation of PSMD14 by siRNA transfection had a considerable impact on cell viability causing cell arrest in the G0-G1 phase, ultimately leading to senescence.

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