Anticorps Recombinant de lapin anti-PSMD3

PSMD3 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242470D4

N° de cat : 84928-2-PBS

Synonymes

242470D4, 26S proteasome non-ATPase regulatory subunit 3, P58, RPN3, S3

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Galerie de données de validation

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  WB analysis using 84928-2-RR (same clone as 84928-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84928-2-RR (PSMD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

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  WB analysis using 84928-2-RR (same clone as 84928-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84928-2-RR (PSMD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84928-2-RR (same clone as 84928-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84928-2-RR (PSMD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84928-2-RR (same clone as 84928-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84928-2-RR (PSMD3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84928-2-RR (same clone as 84928-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMD3 antibody (84928-2-RR, Clone: 242470D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 84928-2-RR (same clone as 84928-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PSMD3 antibody (84928-2-RR, Clone: 242470D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 84928-2-RR (same clone as 84928-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSMD3 antibody (84928-2-RR, Clone: 242470D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 84928-2-RR (same clone as 84928-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using PSMD3 antibody (84928-2-RR, Clone: 242470D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 84928-2-RR (same clone as 84928-2-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug PSMD3 Recombinant antibody (84928-2-RR, Clone:242470D4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84928-2-RR (same clone as 84928-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PSMD3 Recombinant antibody (84928-2-RR, Clone:242470D4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01691-2

  Cytometric bead array standard curve of MP01691-2, PSMD3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84928-2-PBS. Detection antibody: 84928-1-PBS. Standard: Ag2684. Range: 0.781-100 ng/mL.

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Informations sur le produit

84928-2-PBS cible PSMD3 dans les applications de WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PSMD3 Protéine recombinante Ag2684
• Nom complet: proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
• Masse moléculaire calculée: 534 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC012302
• Symbole du gène: PSMD3
• Identification du gène (NCBI): 5709
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PSMD3, encoding proteasome (prosome, macropain) 26S subunit, non-ATPase 3, a member of the 26S proteasome family. PSMD3 is expressed in most of the tissues according to the RNA-Seq database. It has been reported that PSMD3 genotypes are associated with white blood cell (WBC) and neutrophil counts and have interactions with dietary fatty acids and carbohydrates on glucose-related traits. PSMD3 is located at ch17q21 and encodes a protein with a molecular weight of 61 kDa (PMID: 31013812).

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