Anticorps Monoclonal anti-PSMD5

PSMD5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG3

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D5F7

N° de cat : 67682-1-PBS

Synonymes

26S protease subunit S5 basic, 26S proteasome subunit S5B, KIAA0072, PSMD5, S5B

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Galerie de données de validation

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  WB analysis of pig liver using 67682-1-Ig (same clone as 67682-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 67682-1-Ig (same clone as 67682-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

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  WB analysis of HCT 116 using 67682-1-Ig (same clone as 67682-1-PBS)

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit liver using 67682-1-Ig (same clone as 67682-1-PBS)

  rabbit liver tissue were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 67682-1-Ig (same clone as 67682-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67682-1-Ig (same clone as 67682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67682-1-Ig (PSMD5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67682-1-Ig (same clone as 67682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67682-1-Ig (PSMD5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67682-1-Ig (same clone as 67682-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PSMD5 antibody (67682-1-Ig, Clone: 1D5F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67682-1-PBS cible PSMD5 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PSMD5 Protéine recombinante Ag19298
• Nom complet: proteasome (prosome, macropain) 26S subunit, non-ATPase, 5
• Masse moléculaire calculée: 504 aa, 56 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC014478
• Symbole du gène: PSMD5
• Identification du gène (NCBI): 5711
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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