Anticorps Monoclonal anti-PSMD9

• Phare
• Validé par KD/KO

PSMD9 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1H2G1

N° de cat : 67338-1-Ig

Synonymes

1H2G1, 26S proteasome non-ATPase regulatory subunit 9, p27, Rpn4

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Galerie de données de validation

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  WB analysis using 67338-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67338-1-Ig (PSMD9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 67338-1-Ig

  WB result of PSMD9 antibody (67338-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD9 transfected A549 cells.

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  WB analysis using 67338-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67338-1-Ig (PSMD9 antibody) at dilution of 1:2800 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 67338-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSMD9 antibody (67338-1-Ig, Clone: 1H2G1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 67338-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PSMD9 antibody (67338-1-Ig, Clone: 1H2G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 67338-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PSMD9 Monoclonal antibody (67338-1-Ig, Clone:1H2G1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NIH/3T3
• Résultats positifs en IF/ICC: cellules U2OS, cellules HeLa
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

67338-1-Ig cible PSMD9 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PSMD9 Protéine recombinante Ag25654
• Nom complet: proteasome (prosome, macropain) 26S subunit, non-ATPase, 9
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC004213
• Symbole du gène: PSMD9
• Identification du gène (NCBI): 5715
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMD9 is a ubiquitous protein of eukaryotic cells and is a chaperon of the 26S proteasome complex, which degrades ubiquitinated proteins in eukaryotic cells and contributes to the degradation of intracellular proteins into antigenic peptides for antigen presentation by MHC class I cells. The 26S mammalian base sub-complex involves three distinct modules which have ATPase subunits distinctly associated to three chaperones, one of which is PSMD9 regulating the modules assembly. The PSMD9 ubiquitous regulatory role within the proteasome implies its potential pleiotropic effects within different physio-pathological systems. PSMD9 is known to form a stable subcomplex with PSMC3 and PSMC6, two of the AAA-ATPases, assisting in the assembly of the 20S and 19S particles to form the holo complex.

Publications

Species	Application	Title
human	WB,IHC,IF	CNS Neurosci Ther; Comprehensive analysis of PSMD family members and validation of PSMD9 as a potential therapeutic target in human glioblastoma; Authors - Yaquan Li
pig	IF	J Virol; Swine RNF5 positively regulates the antiviral activity of IFITM1 by mediating the degradation of ABHD16A; Authors - Xuemeng Shi

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