Anticorps Polyclonal de lapin anti-PSME1

PSME1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10543-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse spleen using 10543-1-AP (same clone as 10543-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10543-1-AP (PSME1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10543-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 10543-1-AP (same clone as 10543-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 10543-1-AP (PSME1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10543-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 10543-1-AP (same clone as 10543-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10543-1-AP (PSME1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10543-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 10543-1-AP (same clone as 10543-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10543-1-AP (PSME1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10543-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 10543-1-AP (same clone as 10543-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10543-1-AP (PSME1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10543-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10543-1-PBS cible PSME1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSME1 Protéine recombinante Ag0831
• Nom complet: proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 29-33 kDa
• Numéro d’acquisition GenBank: BC007503
• Symbole du gène: PSME1
• Identification du gène (NCBI): 5720
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.

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