Anticorps Monoclonal anti-PSME3

PSME3 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

2A8E8

N° de cat : 67629-1-PBS

Synonymes

Ki, Ki nuclear autoantigen, PA28 gamma, PA28G, PA28gamma, PSME3, REG GAMMA

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Galerie de données de validation

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  WB analysis using 67629-1-Ig (same clone as 67629-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67629-1-Ig (PSME3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67629-1-PBS in a different storage buffer formulation.

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  WB analysis using 67629-1-Ig (same clone as 67629-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67629-1-Ig (PSME3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67629-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67629-1-Ig (same clone as 67629-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67629-1-Ig (PSME3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67629-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67629-1-Ig (same clone as 67629-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67629-1-Ig (PSME3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67629-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67629-1-PBS cible PSME3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PSME3 Protéine recombinante Ag6716
• Nom complet: proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC001423
• Symbole du gène: PSME3
• Identification du gène (NCBI): 10197
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PSME3 gene encodes proteasome activator complex subunit 3, which is also called REG-gamma or PA28-gamma. REG-gamma activates the trypsin-like catalytic subunit of the proteasome thus is a proteasome regulator. MDM2-TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of TP53, may be promoted by REG-gamma resulting in inhibited apoptosis after DNA damage. REG-gamma may also be involved in cell cycle regulation.

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