Anticorps Polyclonal de lapin anti-PSME4

• Phare
• Validé par KD/KO

PSME4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18799-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HEK-293 using 18799-1-AP (same clone as 18799-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18799-1-AP (PSME4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 18799-1-AP (same clone as 18799-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18799-1-AP (PSME4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 18799-1-AP (same clone as 18799-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18799-1-AP (PSME4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 18799-1-AP (same clone as 18799-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18799-1-AP (PSME4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 18799-1-AP (same clone as 18799-1-PBS)

  IP result of anti-PSME4 (IP:18799-1-AP, 3ug; Detection:18799-1-AP 1:500) with HEK-293 cells lysate 1760ug. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 18799-1-AP (same clone as 18799-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18799-1-AP (PSME4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 18799-1-AP (same clone as 18799-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18799-1-AP (PSME4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 18799-1-AP (same clone as 18799-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using PSME4 antibody 18799-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 18799-1-AP (same clone as 18799-1-PBS)

  Immunofluorescent analysis of Hela cells, using PSME4 antibody 18799-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 18799-1-AP (same clone as 18799-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using PSME4 antibody 18799-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18799-1-PBS cible PSME4 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: proteasome (prosome, macropain) activator subunit 4
• Masse moléculaire calculée: 211 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC113668
• Symbole du gène: PSME4
• Identification du gène (NCBI): 23198
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PSME4, like PSME1, 2, and 3, encodes proteasome activator complex subunit 4 also named proteasome activator 200 kDa (PA200). PA200 activates proteasomal cleavage of peptides in an energy-independent manner. After exposure of cells to ionizing radiation, PA200 forms nuclear foci and enhances proteasome activity independent of the stage of cell cycle arrest. PA200 also may be involved in spermatogenesis. The antibody is expected to recognize all the 4 isoforms of PSME4 but appears to mainly detect isoform 2 of 140-kDa.

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