Anticorps Polyclonal de lapin anti-PSMF1

• Phare
• Validé par KD/KO

PSMF1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 12941-1-AP

Synonymes

hPI31, PI31, PSMF1

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Galerie de données de validation

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  WB analysis using 12941-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12941-1-AP (PSMF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12941-1-AP

  WB result of PSMF1 antibody (12941-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMF1 transfected HEK-293 cells.

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  WB analysis of human kidney using 12941-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12941-1-AP (PSMF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12941-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12941-1-AP (PSMF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat, cellules Neuro-2a, tissu cardiaque humain, tissu rénal humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

12941-1-AP cible PSMF1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMF1 Protéine recombinante Ag4011
• Nom complet: proteasome (prosome, macropain) inhibitor subunit 1 (PI31)
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC029836
• Symbole du gène: PSMF1
• Identification du gène (NCBI): 9491
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMF1 is also named as PI31(proteasome (prosome, macropain) inhibitor subunit 1) and belongs to proteasome inhibitor PI31 family. PI31, which localizes at the nuclear envelope/endoplasmic reticulum membrane, acts as a selective modulator of the proteasome-mediated steps in MHC class I antigen processing(PMID:18495667). PI31 does not inhibit cell-surface transport of glycoproteins in general and it may serve to control immunoproteasome formation and may thereby maintain an intracellular balance between constitutive and immunoproteasomes(PMID:12374861).

Publications

Species	Application	Title
human	WB	Mol Cell Biol; Interactome analysis reveals a novel role for RAD6 in the regulation of proteasome activity and localization in response to DNA damage.; Authors - Hongli An
human	WB	Mol Cell; Convergence of orphan quality control pathways at a ubiquitin chain-elongating ligase; Authors - Sara Carrillo Roas

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