Anticorps Monoclonal anti-PSMG3

PSMG3 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

2H9C3

N° de cat : 67466-1-Ig

Synonymes

C7orf48, hPAC3, PAC 3, PAC3, PSMG3

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Galerie de données de validation

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  WB analysis using 67466-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67466-1-Ig (PSMG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67466-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67466-1-Ig (PSMG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of K-562 using 67466-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67466-1-Ig (PSMG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 67466-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67466-1-Ig (PSMG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67466-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67466-1-Ig (PSMG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 67466-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67466-1-Ig (PSMG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 67466-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMG3 antibody (67466-1-Ig, Clone: 2H9C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules A549, cellules HEK-293, cellules HeLa, cellules HL-60, cellules Jurkat, cellules K-562
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67466-1-Ig cible PSMG3 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PSMG3 Protéine recombinante Ag8384
• Nom complet: proteasome (prosome, macropain) assembly chaperone 3
• Masse moléculaire calculée: 13 kDa
• Poids moléculaire observé: 13 kDa
• Numéro d’acquisition GenBank: BC004308
• Symbole du gène: PSMG3
• Identification du gène (NCBI): 84262
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	bioRxiv; PI31 is a positive regulator of 20S immunoproteasome assembly; Authors - Jason Wang

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