Anticorps Polyclonal de lapin anti-PTBP1

• Phare
• Validé par KD/KO

PTBP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

39

N° de cat : 12582-1-AP

Synonymes

HNRNP I, HNRNPI, HNRPI, pPTB, PTB, PTB 1, PTB T, PTB2, PTB3, PTB4, PTBP1

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Galerie de données de validation

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  WB analysis of HeLa using 12582-1-AP

  WB result of PTBP1 antibody (12582-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTBP1 transfected HeLa cells.

• 

  WB analysis using 12582-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12582-1-AP (PTBP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 12582-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 12582-1-AP (PTBP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12582-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12582-1-AP (PTBP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 12582-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12582-1-AP (PTBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12582-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12582-1-AP (PTBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12582-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12582-1-AP (PTBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12582-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12582-1-AP (PTBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 12582-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 12582-1-AP (PTBP1 Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas using 12582-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 12582-1-AP (PTBP1 Antibody) at dilution of 1:50.

• 

  IHC staining of human prostate cancer using 12582-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12582-1-AP (PTBP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of mouse brain using 12582-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12582-1-AP (PTBP1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 12582-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12582-1-AP (PTBP1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules BxPC-3, cellules HeLa, cellules Jurkat, cellules K-562, tissu cardiaque de rat, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu cérébral de souris, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 32 publications below
• IHC: See 3 publications below
• IF: See 8 publications below
• IP: See 2 publications below
• RIP: See 4 publications below

Informations sur le produit

12582-1-AP cible PTBP1 dans les applications de WB, RIP, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTBP1 Protéine recombinante Ag3277
• Nom complet: polypyrimidine tract binding protein 1
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC013694
• Symbole du gène: PTBP1
• Identification du gène (NCBI): 5725
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Polypyrimidine tract-binding protein (PTBP1), also known as heterogeneous nuclear ribonucleoprotein type I (hnRNP I), is a nuclear protein that binds pre-mRNAs in specific regions of the hnRNA-protein complexes sensitive to micrococcal nuclease. It involves in pre-mRNA splicing and the regulation of alternative splicing events. PTBP1 promotes RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA, and promotes the binding of U2 snRNP to pre-mRNA. Besides, it can cooperate with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA, and represses the splicing of MAPT/Tau exon 10

Publications

Species	Application	Title
human	WB	Nat Cancer; SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers; Authors - Lei Shi
human	WB	Nat Cell Biol; Arg-tRNA synthetase links inflammatory metabolism to RNA splicing and nuclear trafficking via SRRM2; Authors - Haissi Cui
human	RIP	Blood; circMYBL2, a circRNA from MYBL2, regulates FLT3 translation by recruiting PTBP1 to promote FLT3-ITD AML progression.; Authors - Yu-Meng Sun
human	WB	Nat Commun; Systematic characterization of cancer transcriptome at transcript resolution; Authors - Wei Hu
rat	WB	Nucleic Acids Res; PMID: 30295850; Authors - Zonghong Li
human	WB	Dev Cell; Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.; Authors - Yanni Ma