Anticorps Monoclonal anti-PTBP1

• Phare
• Validé par KD/KO

PTBP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3D6D8

N° de cat : 67462-1-PBS

Synonymes

3D6D8, HNRNP I, HNRNPI, HNRPI, pPTB

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Galerie de données de validation

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  WB analysis using 67462-1-Ig (same clone as 67462-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67462-1-Ig (PTBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67462-1-Ig (same clone as 67462-1-PBS)

  WB result of PTBP1 antibody (67462-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTBP1 transfected HeLa cells. This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67462-1-Ig (same clone as 67462-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67462-1-Ig (PTBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67462-1-Ig (same clone as 67462-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67462-1-Ig (PTBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67462-1-Ig (same clone as 67462-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67462-1-Ig (PTBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67462-1-Ig (same clone as 67462-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67462-1-Ig (PTBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

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  IF Staining of A375 using 67462-1-Ig (same clone as 67462-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using 67462-1-Ig (PTBP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67462-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67462-1-PBS cible PTBP1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PTBP1 Protéine recombinante Ag28404
• Nom complet: polypyrimidine tract binding protein 1
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC013694
• Symbole du gène: PTBP1
• Identification du gène (NCBI): 5725
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Polypyrimidine tract-binding protein (PTBP1), also known as heterogeneous nuclear ribonucleoprotein type I (hnRNP I), is a nuclear protein that binds pre-mRNAs in specific regions of the hnRNA-protein complexes sensitive to micrococcal nuclease. It involves in pre-mRNA splicing and the regulation of alternative splicing events. PTBP1 promotes RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA, and promotes the binding of U2 snRNP to pre-mRNA. Besides, it can cooperate with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA, and represses the splicing of MAPT/Tau exon 10.

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