Anticorps Polyclonal de lapin anti-PTBP2

• Phare
• Validé par KD/KO

PTBP2 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55186-1-PBS

Synonymes

PTB like protein, PTB, Polypyrimidine tract-binding protein 2, Neurally-enriched homolog of PTB, Neural polypyrimidine tract-binding protein

---

Galerie de données de validation

• 

  WB analysis of mouse heart using 55186-1-AP (same clone as 55186-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 55186-1-AP (same clone as 55186-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 55186-1-AP (same clone as 55186-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 55186-1-AP (same clone as 55186-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 55186-1-AP (same clone as 55186-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 55186-1-AP (same clone as 55186-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 55186-1-AP (same clone as 55186-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 55186-1-AP (same clone as 55186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55186-1-AP (PTBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 55186-1-AP (same clone as 55186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55186-1-AP (PTBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 55186-1-AP (same clone as 55186-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 55186-1-AP (PTBP2 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 55186-1-AP (same clone as 55186-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 55186-1-AP (PTBP2 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

55186-1-PBS cible PTBP2 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: polypyrimidine tract binding protein 2
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57-60 kDa
• Numéro d’acquisition GenBank: NM_021190
• Symbole du gène: PTBP2
• Identification du gène (NCBI): 58155
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PTBP2, also named as NPTB, PTB and PTBLP, is a RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. It may antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. Beside its function in pre-mRNA splicing, PTBP2 plays also a role in the regulation of translation. Isoform 5 has a reduced affinity for RNA. (PMID:11003644) . Protein may exist in a dimeric form with a MW of 110-120 kDa (PMID: 20064465). This antibody is specific to PTBP2.

{{ptg:RelatedPrimaryAntibodies}}