Anticorps Polyclonal de lapin anti-PTCH1

• Phare
• Validé par KD/KO

PTCH1 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

27

Avis (3)

N° de cat : 17520-1-AP

Synonymes

HPE7, NBCCS, Patched, patched homolog 1 (Drosophila), Patched1, Protein patched homolog 1, PTC, PTC1, PTCH, PTCH1, PTCH11

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Galerie de données de validation

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  WB analysis of HeLa using 17520-1-AP

  WB result of PTCH1 antibody (17520-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTCH1 transfected HeLa cells.

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  WB analysis of mouse brain using 17520-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17520-1-AP (PTCH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 17520-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17520-1-AP (PTCH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17520-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17520-1-AP (PTCH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 17520-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17520-1-AP (PTCH1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 17520-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17520-1-AP (PTCH1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A431, cellules HeLa
• Résultats positifs en IHC: tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 21 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

17520-1-AP cible PTCH1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: patched homolog 1 (Drosophila)
• Masse moléculaire calculée: 161 kDa
• Poids moléculaire observé: 161 kDa
• Numéro d’acquisition GenBank: NM_000264
• Symbole du gène: PTCH1
• Identification du gène (NCBI): 5727
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTCH1 (patched homolog 1) is a twelve-pass transmembrane protein that acts as a receptor of Hedgehog signaling pathway (PMID: 8906794). Hedgehog pathway plays a critical role in embryonic development and tissue homeostasis, and its dysregulation has been implicated in many human diseases including congenital disorder and cancer (PMID: 23719536; 23532857). In the absence of Hedgehog proteins (SHH, IHH and DHH in humans), PTCH1 represses the activity of Smoothened (SMO). Binding of Hedgehog proteins to PTCH1 inhibits the repression of SMO, resulting in the activation of downstream targets through the Gli transcriptional effectors (PMID: 23719536; 17139287). The gene of PTCH1 is considered to be a tumor suppressor gene. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly.

Publications

Species	Application	Title
human	WB	Nat Commun; TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.; Authors - Rongxuan Zhu
mouse	WB	Nat Commun; mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation.; Authors - Bo Yan
mouse	IHC	Am J Hum Genet; Genomic analyses reveal mutational signatures and frequently altered genes in esophageal squamous cell carcinoma.; Authors - Ling Zhang
human	WB	Elife; Activation of Hedgehog signaling in mesenchymal stem cells induces cartilage and bone tumor formation via Wnt/β-Catenin.; Authors - Qi Deng
mouse	WB	J Mol Cell Biol; Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression.; Authors - Yuwei Li
human	WB	Cancers (Basel); RNA-seq and ChIP-seq Identification of Unique and Overlapping Targets of GLI Transcription Factors in Melanoma Cell Lines; Authors - Matea Kurtović

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Charlotte (Verified Customer) (07-27-2022)

1/2 : WT starved and then ShhN stimulated. 3/4 : Ptch1 KO cells starved and then ShhN stimulated. It should be between the uppermost band and the following one from the ladder but we can conclude that there is nothing and the antibody is really dirty

• Applications: Western Blot
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: NIH-3T3

PTCH1 Antibody Western Blot validation (1/500 dilution) in NIH-3T3 (Cat no:17520-1-AP)

FH

Boyan (Verified Customer) (04-04-2019)

The band at around 200 KD is not the true band of Ptc1, because this band cannot be increased by treating the cells with Shh ligand, and did not disappear in Ptc1 KO cells. As positive controls, Gli1 was induced by treating the cells with the Shh ligand.

• Applications: Western Blot,

FH

Juan Pablo (Verified Customer) (11-29-2018)

I see a band around 200kDa kind of clean. Never detected the 149kDa shown in proteintech website.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cerebellum from postnatal pups P2 to P14

PTCH1 Antibody Western Blot, validation (1:1000 dilution) in Cerebellum from postnatal pups P2 to P14 (Cat no:17520-1-AP)

Produits associés

SHH Polyclonal antibody

20697-1-AP

PTCH2 Polyclonal antibody

55091-1-AP

IHH Polyclonal antibody

13388-1-AP

DHH Polyclonal antibody

13889-1-AP

SMO Polyclonal antibody

20787-1-AP